Single DNA molecule analysis in hospital labs

Next-Generation Sequencing techniques are unsuitable for diagnosing certain inherited diseases and blood cancers due to the need to fragment the genome, making certain abnormalities e.g., translocations difficult to measure.


Prof. Reed at Virginia Commonwealth University successfully used a previous D-SPM version on existing clinical samples from various trials to accurately identify these abnormalities in CRISPR-labelled genomic DNA. This analysis fitted into the existing analysis workflows for the samples’ respective trials, and was accomplished by lab technicians – users unfamiliar with D-SPM – with little to no manual intervention required for set up and analysis.